Well, I tried to help Datu with the pump and that did not go so well. But, Tanya might want to test for phosphate. Fungi, virus and Bacteria will have it. She might not be able to get them to grow, but if she does not find Phosphate, then she does not have a life form. It could still be a prion, or a chemical, but without phosphate, it is not life as we know it, but that just begs the question was it spewing fungus or virus or flying squid at some other time...